The majority of harmful or illegal drugs alter the person's perception and personality, some temporarily and some permanently. Energy Steroids main real-world influence, steroids, does account for the users being more alert and confident, to paranoid and aggressive, with many mixing both wanted and unwanted personality traits. Since Hody developed such a high tolerance that he needed to practically shovel them into his mouth to achieve the same effects (and null the pain from the grievous injuries sustained from his fight with Luffy), the adverse effects of the drug soon caught up to him, with their fatal side effects increased exponentially to parallel his increase in muscle mass and aggression.
Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Despite his love for excessive violence being aimed towards bandits and criminals, Salvador was nonetheless about to be executed by the people of his own hometown of Ovejas for his actions when a Hyperion strike team arrived to seize the town. After destroying the invading forces, Salvador becomes interested in the new Vault upon hearing of its dangers while "interrogating" the last survivor of the strike team and leaving him to crawl back to Hyperion on a single arm (as Salvador had ripped off his other arm and broken both his legs).