This research was conducted with the maceration extraction continuing by test toxicity against the shrimp larvae of A . salina Leach (BSLT). The result of the toxicity test then proceeds with the test reagents and phytochemicals with thin layer chromatography for positive test of compound phytochemicals. Thin-layer chromatography using eluen n-hexane: ethyl acetate (7: 3) to ethanol extract and n-hexane: ethyl acetate (17: 3) for n-hexane extract. Data on toxicity of A. salina Leach analyzed by probit analysis to find out the value of LC 50 on each extract.
The study about the growth and qualitative test of secondary metabolite content of the callus culture of Spilanthes acmella Murr. with addition of PEG to induce drought stress had been done used completely randomized design with six treatments and six replications. The treatments were the addition of PEG in various consentration : 1%, 2%, 3%, 4%, 5% and control (without addition of PEG). The result showed that the addition of PEG to medium could decrease fresh weight of callus. The fresh weight of callus was decrease significantly by addition 5% PEG. On the qualitative test of secondary metabolite, alkaloid content was increase by addition of 2% - 5% PEG (++), terpenoid content was increase by addition 3% - 4% PEG (++) and fenolik was found on 4% PEG (+).